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Issue 11, 2017
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Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over a dynamic range of 6 logs and beyond

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Abstract

Standard digital assays need a large number of compartments for precise quantification of a sample over a broad dynamic range. We address this issue with an optimized droplet digital approach that uses a drastically reduced number of compartments for quantification. We generate serial logarithmic dilutions of an initial bacterial sample as an array of microliter-sized droplet plugs. In a subsequent step, these droplets are split into libraries of nanoliter droplets and pooled together for incubation and analysis. We show that our technology is at par with traditional dilution plate count for quantification of bacteria, but has the advantage of simplifying the experimental setup and reducing the manual workload. The method also has the potential to reduce the assay time significantly.

Graphical abstract: Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over a dynamic range of 6 [ ] logs and beyond

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Publication details

The article was received on 28 Feb 2017, accepted on 26 Apr 2017 and first published on 28 Apr 2017


Article type: Paper
DOI: 10.1039/C7LC00206H
Citation: Lab Chip, 2017,17, 1980-1987
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    Optimized droplet digital CFU assay (ddCFU) provides precise quantification of bacteria over a dynamic range of 6 logs and beyond

    O. Scheler, N. Pacocha, P. R. Debski, A. Ruszczak, T. S. Kaminski and P. Garstecki, Lab Chip, 2017, 17, 1980
    DOI: 10.1039/C7LC00206H

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