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Droplet-based light-sheet fluorescence microscopy for high-throughput sample preparation, 3-D imaging and quantitative analysis on a chip

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Abstract

We report a novel fusion of droplet microfluidics and light-sheet microscopy, to achieve high-throughput sample compartmentalization, manipulation and three-dimensional imaging on a chip. This optofluidic device characterized by orthogonal plane illumination and rapid liquid handling is compact and cost-effective, and capable of preparing sample droplets with tunable size, frequency and ingredient. Each droplet flowing through the device's imaging region is self-scanned by a laser-sheet, three-dimensionally reconstructed and quantitatively analysed. This simple-and-robust platform combines fast 3-D imaging with efficient sample preparation and eliminates the need of a complicated mechanical scan at the same time. Achieving 500 measurements per second and screening over 30 samples per minute, it shows great potential for various lab-on-a-chip biological studies, such as embryo sorting and cell growth assays.

Graphical abstract: Droplet-based light-sheet fluorescence microscopy for high-throughput sample preparation, 3-D imaging and quantitative analysis on a chip

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Publication details

The article was received on 17 Feb 2017, accepted on 04 Jun 2017 and first published on 06 Jun 2017


Article type: Communication
DOI: 10.1039/C7LC00164A
Citation: Lab Chip, 2017, Advance Article
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    Droplet-based light-sheet fluorescence microscopy for high-throughput sample preparation, 3-D imaging and quantitative analysis on a chip

    H. Jiang, T. Zhu, H. Zhang, J. Nie, Z. Guan, C. Ho, S. Liu and P. Fei, Lab Chip, 2017, Advance Article , DOI: 10.1039/C7LC00164A

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