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Issue 7, 2017
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Micro vapor bubble jet flow for safe and high-rate fluorescence-activated cell sorting

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Abstract

Safe, high-rate and cost-effective cell sorting is important for clinical cell isolation. However, commercial fluorescence-activated cell sorters (FACS) are expensive and prone to aerosol-induced sample contamination. Here we report a microfluidic cell sorter allowing high rate and fully enclosed cell sorting. The sorter chip consists of an array of micro heating hotspots. Pulsed resistive heating in the hotspots produces numerous micro vapor bubbles with short duration, which gives rise to a rapid jet flow for cell sorting. With this method, we demonstrated high sorting rate comparable to commercial FACS and the significant enrichment of rare cancer cells. This vapor bubble based cell sorting method can be a powerful tool for contamination-free and affordable clinical cell sorting such as circulating tumor cell isolation and cancer cell therapy.

Graphical abstract: Micro vapor bubble jet flow for safe and high-rate fluorescence-activated cell sorting

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Publication details

The article was received on 20 Dec 2016, accepted on 23 Feb 2017 and first published on 23 Feb 2017


Article type: Paper
DOI: 10.1039/C6LC01560C
Citation: Lab Chip, 2017,17, 1287-1296
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    Micro vapor bubble jet flow for safe and high-rate fluorescence-activated cell sorting

    K. de Wijs, C. Liu, A. Dusa, D. Vercruysse, B. Majeed, D. S. Tezcan, K. Blaszkiewicz, J. Loo and L. Lagae, Lab Chip, 2017, 17, 1287
    DOI: 10.1039/C6LC01560C

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