Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.



Fabrication of large-area polymer microfilter membranes and their application for particle and cell enrichment

Author affiliations

Abstract

A vacuum assisted UV micro-molding (VAUM) process is proposed for the fabrication of freestanding and defect-free polymer membranes based on a UV-curable methacrylate polymer (MD 700). VAUM is a highly flexible and powerful method for fabricating low cost, robust, large-area membranes over 9 × 9 cm2 with pore sizes from 8 to 20 μm in diameter, 20 to 100 μm in thickness, high aspect ratio (the thickness of the polymer over the diameter of the hole is up to 15 : 1), high porosity, and a wide variety of geometrical characteristics. The fabricated freestanding membranes are flexible while mechanically robust enough for post manipulation and handling, which allows them to be cut and integrated as a plastic cartridge onto thermoplastic 3D microfluidic devices with single or double filtration stages. Very high particle capture efficiencies (≈98%) have been demonstrated in the microfluidic devices integrated with polymer membranes, even when the size of the beads is very close to the size of the pores of the microfilter. About 85% of the capture efficiency has been achieved in cancer cell trapping experiments, in which a breast cancer cell line (MDA-MB-231) spiked with phosphate-buffered saline buffer when the pore size of the filter is 8 μm and the device is operated at a flow rate of 0.1 mL min−1.

Graphical abstract: Fabrication of large-area polymer microfilter membranes and their application for particle and cell enrichment

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 13 Dec 2016, accepted on 13 Apr 2017 and first published on 13 Apr 2017


Article type: Paper
DOI: 10.1039/C6LC01525E
Citation: Lab Chip, 2017, Advance Article
  •   Request permissions

    Fabrication of large-area polymer microfilter membranes and their application for particle and cell enrichment

    J. A. Hernández-Castro, K. Li, A. Meunier, D. Juncker and T. Veres, Lab Chip, 2017, Advance Article , DOI: 10.1039/C6LC01525E

Search articles by author