Jump to main content
Jump to site search

Issue 5, 2017
Previous Article Next Article

A microfluidic device for characterizing nuclear deformations

Author affiliations

Abstract

Cell nuclei experience and respond to a wide range of forces, both in vivo and in vitro. In order to characterize the nuclear response to physical stress, we developed a microfluidic chip and used it to apply mechanical stress to live cells and measure their nuclear deformability. The device design is optimized for the detection of both nucleus and cytoplasm, which can then be conveniently quantified using a custom-written Matlab program. We measured nuclear sizes and strains of embryonic stem cells, for which we observed negative Poisson ratios in the nuclei. In addition, we were able to detect changes in the nuclear response after treatment with actin depolymerizing and chromatin decondensing agents. Finally, we showed that the device can be used for biologically relevant high-resolution confocal imaging of cells under compression. Thus, the device presented here allows for accurate physical phenotyping at high throughput and has the potential to be applied to a range of cell types.

Graphical abstract: A microfluidic device for characterizing nuclear deformations

Back to tab navigation

Publication details

The article was received on 21 Oct 2016, accepted on 13 Jan 2017 and first published on 13 Jan 2017


Article type: Paper
DOI: 10.1039/C6LC01308B
Citation: Lab Chip, 2017,17, 805-813
  •   Request permissions

    A microfluidic device for characterizing nuclear deformations

    A. C. Hodgson, C. M. Verstreken, C. L. Fisher, U. F. Keyser, S. Pagliara and K. J. Chalut, Lab Chip, 2017, 17, 805
    DOI: 10.1039/C6LC01308B

Search articles by author

Spotlight

Advertisements