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Issue 7, 2017
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Isolation and concentration of bacteria from blood using microfluidic membraneless dialysis and dielectrophoresis

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Abstract

A microfluidic system that combines membraneless microfluidic dialysis and dielectrophoresis to achieve label-free isolation and concentration of bacteria from whole blood is presented. Target bacteria and undesired blood cells are discriminated on the basis of their differential susceptibility to permeabilizing agents that alter the dielectrophoretic behavior of blood cells but not bacteria. The combined membraneless microdialysis and dielectrophoresis system isolated 79 ± 3% of Escherichia coli and 78 ± 2% of Staphylococcus aureus spiked into whole blood at a processing rate of 0.6 mL h−1. Collection efficiency was independent of the number of target bacteria up to 105 cells. Quantitative PCR analysis revealed that bacterial 16S rDNA levels were enriched more than 307-fold over human DNA in the fraction recovered from the isolation system compared with the original specimen. These data demonstrate feasibility for an instrument to accelerate the detection and analysis of bacteria in blood by first isolating and concentrating them in a microchamber.

Graphical abstract: Isolation and concentration of bacteria from blood using microfluidic membraneless dialysis and dielectrophoresis

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Publication details

The article was received on 14 Oct 2016, accepted on 28 Feb 2017, published on 28 Feb 2017 and first published online on 28 Feb 2017


Article type: Paper
DOI: 10.1039/C6LC01277A
Citation: Lab Chip, 2017,17, 1340-1348
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    Isolation and concentration of bacteria from blood using microfluidic membraneless dialysis and dielectrophoresis

    L. D'Amico, N. J. Ajami, J. A. Adachi, P. R. C. Gascoyne and J. F. Petrosino, Lab Chip, 2017, 17, 1340
    DOI: 10.1039/C6LC01277A

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