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Issue 2, 2017
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On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening

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Abstract

Biological assays often require expensive reagents and tedious manipulations. These shortcomings can be overcome using digitally operated microfluidic devices that require reduced sample volumes to automate assays. One particular challenge is integrating bioassays with mass spectrometry based analysis. Towards this goal we have developed μNIMS, a highly sensitive and high throughput technique that integrates droplet microfluidics with nanostructure-initiator mass spectrometry (NIMS). Enzyme reactions are carried out in droplets that can be arrayed on discrete NIMS elements at defined time intervals for subsequent mass spectrometry analysis, enabling time resolved enzyme activity assay. We apply the μNIMS platform for kinetic characterization of a glycoside hydrolase enzyme (CelE-CMB3A), a chimeric enzyme capable of deconstructing plant hemicellulose into monosaccharides for subsequent conversion to biofuel. This study reveals NIMS nanostructures can be fabricated into arrays for microfluidic droplet deposition, NIMS is compatible with droplet and digital microfluidics, and can be used on-chip to assay glycoside hydrolase enzyme in vitro.

Graphical abstract: On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening

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Publication details

The article was received on 20 Sep 2016, accepted on 02 Dec 2016 and first published on 05 Dec 2016


Article type: Paper
DOI: 10.1039/C6LC01182A
Citation: Lab Chip, 2017,17, 323-331
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    On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening

    J. Heinemann, K. Deng, S. C. C. Shih, J. Gao, P. D. Adams, A. K. Singh and T. R. Northen, Lab Chip, 2017, 17, 323
    DOI: 10.1039/C6LC01182A

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