Issue 2, 2017

Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy

Abstract

Understanding the properties of protein-based therapeutics is a common goal of biologists and physicians. Technical barriers in the direct observation of small proteins or therapeutic agents can limit our knowledge of how they function in solution and in the body. Electron microscopy (EM) imaging performed in a liquid environment permits us to peer into the active world of cells and molecules at the nanoscale. Here, we employ liquid cell EM to directly visualize a protein-based therapeutic in its native conformation and aggregate state in a time-resolved manner. In combination with quantitative analyses, information from this work contributes new molecular insights toward understanding the behaviours of immunotherapies in a solution state that mimics the human body.

Graphical abstract: Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy

Supplementary files

Article information

Article type
Paper
Submitted
15 Sep 2016
Accepted
01 Dec 2016
First published
05 Dec 2016

Lab Chip, 2017,17, 315-322

Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy

L. M. DiMemmo, A. Cameron Varano, J. Haulenbeek, Y. Liang, K. Patel, M. J. Dukes, S. Zheng, M. Hubert, S. P. Piccoli and D. F. Kelly, Lab Chip, 2017, 17, 315 DOI: 10.1039/C6LC01160H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements