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Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Multi-Elemental Determination in Dried Blood Spots

Abstract

An approach by laser ablation (LA) coupled with inductively coupled mass spectrometry (ICP-MS) for multi-element determination in whole blood is described. The method allows quantitative determination of several major (K, Na and S), minor (Ca, Cu, Fe, Mg, P, Rb and Zn), and trace elements (Al, As, Ba, I, Li, Pb, Sb, V and Tl) in dried blood spot (DBS) samples. Optimum conditions and validation were assessed using SeronormTM level II and SeronormTM level III certified reference materials. Laser ablation of DBS was performed using a focused Nd:YAG laser beam in lineal scan mode (wavelength 213 nm, laser fluency 3.0 J cm−2, repetition rate 20 Hz, laser spot diameter 90 µm, depth 0 µm, scanning speed 8 µm s-1,). Different quantification strategies for the LA-ICP-MS data using matrix-matched calibration or prearranged external calibration / addition set were applied. 13C and 32S were used for signal intensities normalization. Using a matrix-matched calibration curve and 13C or 32S as internal standards normalization, limits of quantification were found to be from 1.9 µg L−1 to 1094 mg L−1. Accuracy (analysis of SeronormTM level II) was satisfactory after statistical evaluation by applying a t-test at 95% confidence level. Repeatability and reproducibility of the methods were generally below 14%. The method was applied to several DBS from newborns

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Publication details

The article was received on 28 Apr 2017, accepted on 08 Jun 2017 and first published on 08 Jun 2017


Article type: Paper
DOI: 10.1039/C7JA00168A
Citation: J. Anal. At. Spectrom., 2017, Accepted Manuscript
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    Laser Ablation Inductively Coupled Plasma Mass Spectrometry for Multi-Elemental Determination in Dried Blood Spots

    J. Moreda-Piñeiro, A. Cantarero-Roldán, A. Moreda-Pineiro, J. A. Cocho and P. Bermejo-Barrera, J. Anal. At. Spectrom., 2017, Accepted Manuscript , DOI: 10.1039/C7JA00168A

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