Jump to main content
Jump to site search

Issue 5, 2017
Previous Article Next Article

Visualisation of developmental ossification using trace element mapping

Author affiliations

Abstract

Endochondral ossification is the process by which bone is deposited during development, growth and repair of the skeleton. The regulation of endochondral ossification is extremely important as developmental flaws can result in severe skeletal abnormalities. However, until recently the limitations of available methodologies have restricted our understanding of this fundamental physiological process. The analysis of chemical elements that are intimately associated with discrete biochemical stages of ossification within bone could provide new insight to such processes at the atomic level. In this study we present detailed characterisation of the elemental inventory within actively ossifying bone during development in mice using synchrotron microfocus X-ray techniques. X-ray fluorescence imaging showed differential distributions of Zn, Sr and Ca, which may be correlated with the processes of cartilage replacement (Zn), active ossification (Sr) and fully ossified tissues (Ca). Quantification of these trace elements confirmed their relative distributions. These results represent the first detailed visualisation of local endochondral ossification processes using trace elemental mapping. Such studies have far reaching applications not only in the medical field, but to our understanding of the evolution of the bony skeleton given that trace element inventories have been shown to be preserved through deep time (millions of years).

Graphical abstract: Visualisation of developmental ossification using trace element mapping

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Jan 2017, accepted on 15 Mar 2017 and first published on 21 Mar 2017


Article type: Paper
DOI: 10.1039/C7JA00042A
Citation: J. Anal. At. Spectrom., 2017,32, 967-974
  •   Request permissions

    Visualisation of developmental ossification using trace element mapping

    J. Anné, N. P. Edwards, A. van Veelen, V. M. Egerton, P. L. Manning, J. F. W. Mosselmans, S. Parry, W. I. Sellers, M. Buckley and R. A. Wogelius, J. Anal. At. Spectrom., 2017, 32, 967
    DOI: 10.1039/C7JA00042A

Search articles by author

Spotlight

Advertisements