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Modulation of cellular polarization and migration by ephrin/Eph signal-mediated boundary formation

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Abstract

Compartment boundaries are essential for ensuring proper cell organization during embryo development and in adult tissues, yet the mechanisms underlying boundary establishment are not completely understood. A number of mechanisms, including (i) differential adhesion, (ii) differential tension, and (iii) cell signaling-mediated cell repulsion, are known to contribute and likely a context-dependent balance of each of these dictates boundary implementation. The ephrin/Eph signaling pathway is known to impact boundary formation in higher animals. In different contexts, ephrin/Eph signaling is known to modulate adhesive properties and migratory behavior of cells. Furthermore it has been proposed that ephrin/Eph signaling may modulate cellular tensile properties, leading to boundary implementation. It remains unclear however, whether, in different contexts, ephrin/Eph act through distinct dominant action modes (e.g. differential adhesion vs. cell repulsion), or whether ephrin/Eph signaling elicits multiple cellular changes simultaneously. Here, using micropatterning of cells over-expressing either EphB3 or ephrinB1, we assess the contribution of each these factors in one model. We show that in this system ephrinB1/EphB3-mediated boundaries are accompanied by modulation of tissue-level architecture and polarization of cell migration. These changes are associated with changes in cell shape and cytoskeletal organization also suggestive of altered cellular tension.

Graphical abstract: Modulation of cellular polarization and migration by ephrin/Eph signal-mediated boundary formation

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Publication details

The article was received on 10 Oct 2017, accepted on 01 Nov 2017 and first published on 02 Nov 2017


Article type: Paper
DOI: 10.1039/C7IB00176B
Citation: Integr. Biol., 2017, Advance Article
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    Modulation of cellular polarization and migration by ephrin/Eph signal-mediated boundary formation

    S. Javaherian, E. D’Arcangelo, B. Slater, C. Londono, B. Xu and A. P. McGuigan, Integr. Biol., 2017, Advance Article , DOI: 10.1039/C7IB00176B

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