Jump to main content
Jump to site search

Probing impaired neurogenesis in human brain organoids exposed to alcohol


The fetal brain is highly vulnerable to ethanol exposure, which can trigger various long-term neuronal disabilities and cognitive dysfunctions. However, a comprehensive understanding of fetal brain development under ethanol exposure is challenging due to the limitations of animal models. Here, we propose a human induced pluripotent stem cell (hiPSC)-based 3D brain organoid model, and explore the mechanisms underlying neural dysfunctions in prenatal alcohol exposure (PAE) in vitro. Brain organoids were examined to resemble brain organogenesis in vivo at early stages during gestation, with specific features of neuronal differentiation, brain regionalization, and cortical organization. With ethanol exposure, the brain organoids displayed attenuated neurite outgrowth and skewed neural maturation. Transcriptome analysis identified a series of new markedly altered genes and enriched pathways, such as GSX2, RSPO2, and Hippo signaling pathway. These genes or pathways, to our knowledge, were firstly reported to be involved in ethanol-induced impaired neurogenesis. Our new findings might facilitate better understanding of the various postnatal neural disorders observed in individuals with PAE.

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Jun 2017, accepted on 31 Oct 2017 and first published on 09 Nov 2017

Article type: Paper
DOI: 10.1039/C7IB00105C
Citation: Integr. Biol., 2017, Accepted Manuscript
  •   Request permissions

    Probing impaired neurogenesis in human brain organoids exposed to alcohol

    J. Qin, Y. Zhu, L. Wang, F. Yin, Y. Yu, Y. Wang, M. Shepard and Z. Zhuang, Integr. Biol., 2017, Accepted Manuscript , DOI: 10.1039/C7IB00105C

Search articles by author