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Inducible Bcl-2 gene RNA interference mediated by aptamer-integrated HDV ribozyme switch

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Abstract

The regulation of RNA interference (RNAi) could be a powerful method for the study of temporal and dose dependent effects of gene expression. In this study, we designed the hepatitis delta virus (HDV) ribozyme with an embedded theophylline aptamer as the sensor domain and the pri-miRNA of endogenous gene Bcl-2 as the effector domain to engineer an RNAi-regulatory device in MCF-7 cells. The system allowed us to control gene expression by adding theophylline into the culture media in a dose dependent fashion. This is the pioneering application of ribozyme switches to activate RNAi for modulating endogenous genes in mammalian cells. The platform sets the stage for investigations of other endogenous genes that regulate various biological functions such as differentiation, cell division or cell death, and provides a promising interface with other universal RNAi-based decision-making circuits that operate in mammalian cells. It can be used to study more genes associated with cancer and screen for potential drug targets for gene therapy.

Graphical abstract: Inducible Bcl-2 gene RNA interference mediated by aptamer-integrated HDV ribozyme switch

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Publication details

The article was received on 15 Feb 2017, accepted on 07 May 2017 and first published on 11 May 2017


Article type: Paper
DOI: 10.1039/C7IB00029D
Citation: Integr. Biol., 2017, Advance Article
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    Inducible Bcl-2 gene RNA interference mediated by aptamer-integrated HDV ribozyme switch

    Y. Zhang, J. Wang, H. Cheng, N. Sun, M. Liu, Z. Wu and R. Pei, Integr. Biol., 2017, Advance Article , DOI: 10.1039/C7IB00029D

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