Jump to main content
Jump to site search

Issue 3, 2017
Previous Article Next Article

A finite-element model of granular serotonin exocytosis

Author affiliations

Abstract

Signaling molecules stored in cellular granules serve a broad range of functional purposes. Serotonin, for example, is involved in the regulation of behavior, cognitive function, and hemostasis. Understanding serotonin release from platelet granules can provide valuable fundamental information about the exocytotic process and help elucidate potential abnormalities of diseased platelets. Experimentally, carbon-fiber microelectrochemistry has been utilized to characterize the dynamic behavior of serotonin secretion from individual platelets. The objectives of this study were (1) to develop a finite-element model of the carbon-fiber microelectrode experiment and (2) to predict the effect of the postulated core–halo granule microstructure and rate of fusion pore expansion on the resulting amperometric signal. The model confirmed that the core–halo microstructure is consistent with the observed amperometric spike profiles. Further, it showed that the ratio of pore diameter to granule diameter, rather than the rate of pore expansion, dictates the profile shape. Understanding the driving forces for chemical messenger delivery from platelet granules has implications for understanding abnormalities in diseased platelets as well as other exocytotic cells.

Graphical abstract: A finite-element model of granular serotonin exocytosis

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 18 Nov 2016, accepted on 22 Jan 2017 and first published on 27 Jan 2017


Article type: Paper
DOI: 10.1039/C6IB00226A
Citation: Integr. Biol., 2017,9, 248-256
  •   Request permissions

    A finite-element model of granular serotonin exocytosis

    A. Datta, C. L. Haynes and V. H. Barocas, Integr. Biol., 2017, 9, 248
    DOI: 10.1039/C6IB00226A

Search articles by author