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Synergistic Maximization of Carbohydrate Output and Lignin Processibility by Combinatorial Pretreatment

Abstract

Lignocellulosic biorefineries have gained much attention worldwide as a potential solution to the challenges of energy demand and global climate change. However, the industrial implementation of biorefinery has been hindered by low fermentable sugar yields and low lignin processibility. Combinatorial pretreatments with a low holding temperature were investigated in an effort to synergistically improve the carbohydrate output and lignin processibility from corn stover. Upon combinatorial pretreatment with 1% H2SO4 for 30 min followed by 1% NaOH for 60 min at 120°C, glucan and xylan conversion increased by 11.2% and 8.3% relative to single pretreatment. This combinational pretreatment removed the amorphous portion, disrupted the rigid structure, and increased water holding capacity of corn stover, thus increasing the hydrolysis performance. With whole fractionation by combinatorial pretreatment, glucose and xylose yields were 88.4% and 72.6%, respectively, representing increases of 10.0% and 8.1%. Lignin yield was 19.7% in solid residue and 77.6% in liquid stream, which increased by 33.4%. When grown in fed-batch fermentation mode, a record level of polyhydroxyalkanoate (PHA) concentration (1.0 g/l) was obtained using lignin as carbon sources by Pseudomonas putida KT2440. Lignin characterization results showed that combinatorial pretreatment increased the G- and H- lignin content, reduced the β-β and β-O-4 groups, and fractionated more aromatic monomers, thus facilitating lignin processibility into PHA. These results highlighted the use of combinational pretreatment at low holding temperature as a means to synergistically maximize carbohydrate output and lignin processibility, which provides a unique set of features to improve biorefining performance.

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Supplementary files

Publication details

The article was received on 07 Jul 2017, accepted on 06 Sep 2017 and first published on 07 Sep 2017


Article type: Paper
DOI: 10.1039/C7GC02057K
Citation: Green Chem., 2017, Accepted Manuscript
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    Synergistic Maximization of Carbohydrate Output and Lignin Processibility by Combinatorial Pretreatment

    Z. Liu, M. Olson, S. D. Shinde, X. Wang, N. Hao, C. G. Yoo, S. Bhagia , J. R. Dunlap , Y. Pu, K. Kao, A. Ragauskas, M. Jin and J. S. Yuan, Green Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7GC02057K

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