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Issue 2, 2017
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Design of recombinant whole-cell catalysts for double reduction of C[double bond, length as m-dash]C and C[double bond, length as m-dash]O bonds in enals and application in the synthesis of Guerbet alcohols as industrial bulk chemicals for lubricants

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Abstract

Whole-cell catalysts overexpressing two enzymes for a double reduction cascade in which aliphatic α-branched α,β-unsaturated aldehydes are converted into Guerbet alcohols as a highly demanded class of lubricants were constructed and characterized. The reaction conditions were optimized to suppress by-product formation, achieving high conversion and selectivity. Application of methanol as a co-solvent on a 500 mL scale enabled a substrate concentration of 18.5 g L−1 obtaining 97% yield and 88% purity without further distillation. Addressing the needs of industrial processes, substrate loading was increased up to 94 g L−1 and the E-factor (environmental factor) was significantly improved compared to the original process with isolated enzymes.

Graphical abstract: Design of recombinant whole-cell catalysts for double reduction of C [[double bond, length as m-dash]] C and C [[double bond, length as m-dash]] O bonds in enals and application in the synthesis of Guerbet alcohols as industrial bulk chemicals for lubricants

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Publication details

The article was received on 18 Jun 2016, accepted on 08 Aug 2016 and first published on 06 Sep 2016


Article type: Paper
DOI: 10.1039/C6GC01668E
Citation: Green Chem., 2017,19, 405-410
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    Design of recombinant whole-cell catalysts for double reduction of C[double bond, length as m-dash]C and C[double bond, length as m-dash]O bonds in enals and application in the synthesis of Guerbet alcohols as industrial bulk chemicals for lubricants

    M. Biermann, D. Bakonyi, W. Hummel and H. Gröger, Green Chem., 2017, 19, 405
    DOI: 10.1039/C6GC01668E

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