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Capsaicin and nonivamide similarly modulate outcome measures of mitochondrial energy metabolism in HepG2 and 3T3-L1 cells

Abstract

Capsaicin, the highly pungent principle of red pepper, has been demonstrated to unfold anti-obesity properties by affecting energy and lipid metabolism. Recent evidence from human intervention trials shows that also less pungent capsaicin analogs, like nonivamide, may help to reduce total body fat, although mechanistic data comparing the effects of capsaicin and nonivamide on outcome measures of energy metabolism is lacking. Here, the tissue-specific effects of capsaicin and nonivamide on parameters of mitochondrial energy metabolism in 3T3 L1 and HepG2 cells were investigated. Lipid accumulation was reduced to a similar extent after treatment with both test substances during maturation of 3T3-L1 cells by up to 6.91 % for capsaicin and up to 4.89 % for nonivamide (p<0.01) at a concentration of 0.1 µM or 1 µM, respectively. Energy-producing pathways, as indicated by reduced mitochondrial oxygen consumption and reduced glucose and fatty acid uptake, were diminished after incubation with both capsaicinoids at a concentration of 100 µM. Results from HPLC analyses revealed a reduced cellular energy charge potential after a 4 h treatment with nonivamide. In HepG2 cells, similar effects were demonstrated: glucose uptake was reduced by 18.7 % and 25.8 % (p<0.05), after a 24 h incubation with 100 µM capsaicin and nonivamide, respectively. In addition, fatty acid uptake and oxygen consumption were decreased and energy charge potential was diminished. These findings provide evidence that concentrations of capsaicin and nonivamide between 0.1 – 100 µM modulate mechanisms of cellular energy metabolism to a similar extent, independent of the investigated tissue.

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Publication details

The article was received on 17 Oct 2017, accepted on 12 Dec 2017 and first published on 13 Dec 2017


Article type: Paper
DOI: 10.1039/C7FO01626C
Citation: Food Funct., 2017, Accepted Manuscript
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    Capsaicin and nonivamide similarly modulate outcome measures of mitochondrial energy metabolism in HepG2 and 3T3-L1 cells

    C. M. Hochkogler, B. Lieder, D. Schachner, E. Heiss, A. Schröter, J. Hans, J. Ley, G. E. Krammer and V. Somoza, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01626C

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