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Protective effects of Ganoderma atrum polysaccharide against acrylamide Induced oxidative damage via a mitochondria mediated intrinsic apoptotic pathway in IEC-6 cells

Abstract

The preventive role of a purified Ganoderma atrum polysaccharide PSG-1-F2 as a new dietary antioxidant against the intestinal toxicity of acrylamide (ACR) were investigated in vitro. Our results showed that ACR could induce oxidative stress in IEC-6 cells by overproduction of the reactive oxygen species (ROS) and malondialdehyde (MDA), and as well as reduction in activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px). In addition, induction of mitochondria-mediated intrinsic apoptotic pathway by ACR was evidenced by the events of loss of mitochondrial membrane potential, bax/bcl-2 dysregulation, cytochrome c release, and activation of caspases 3. Interestingly, PSG-1-F2 was able to suppress ACR toxicity by improving the redox status of IEC-6 cells and by attenuating mitochondria-mediated apoptosis. Its protective effect was even superior to the clinically used antioxidant N-acetylcysteine (NAC). This study uniquely introduces PSG-1-F2 as a potential inhibitor of ACR -induced stress and toxicities.

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Publication details

The article was received on 17 Oct 2017, accepted on 23 Dec 2017 and first published on 27 Dec 2017


Article type: Paper
DOI: 10.1039/C7FO01619K
Citation: Food Funct., 2017, Accepted Manuscript
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    Protective effects of Ganoderma atrum polysaccharide against acrylamide Induced oxidative damage via a mitochondria mediated intrinsic apoptotic pathway in IEC-6 cells

    G. Jiang, L. Zhang, H. Wang, Q. Chen, X. Wu, X. Yan, Y. Chen and M. Xie, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01619K

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