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Quercetin and fisetin enhanced small intestine cellular uptake and plasma levels of epi-catechins in vitro and in vivo model

Abstract

Quercetin and fisetin known as catechol-containing flavonoids could positively affect absorption of catechins due to their strong affinity to catechol-O-methyl transferase (COMT), which can methylate and excrete catechins. The current study examined the effect of quercetin and fisetin on epi-catechins (ECs) absorption by using a Caco-2 cell and an in vivo study. Intestinal transport of total catechins by Caco-2 cell was enhanced from 1.3 to 1.6-fold and 1.4 to 1.7-fold by adding quercetin and fisetin, respectively, compared to the control. It was even higher in the mixture of quercetin and fisetin treatment. While EC had the highest value of intestinal transport (169% of control) in 10% quercetin treatment, EGC (235%), EGCG (244%), and ECG (242%) were significantly transported at 5% mixture of quercetin and fisetin (p<0.05). In in vivo pharmacokinetic study, the area under plasma concentration-time curve (AUC, ng*h/mL) were also higher in rats orally administered EGCG with 10% quercetin (365.5±25.5) or 10% fisetin (825.3±46.7) than those with EGCG single intake (111.3±13.1). Methylated quercetin and methylated fisetin was determined (m/z) 317.24 and m/z 301.25 [M+H]+ with their own product ions, respectively. Results indicate quercetin or fisetin is superior to ECs for methylation by COMT.

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Publication details

The article was accepted on 04 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7FO01576C
Citation: Food Funct., 2017, Accepted Manuscript
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    Quercetin and fisetin enhanced small intestine cellular uptake and plasma levels of epi-catechins in vitro and in vivo model

    J. Chung, S. Lee, K. Jeong , J. Song, S. Kim, K. Joo, H. W. Jeong, J. Choi, J. K. Kim, W. G. Kim, S. Seok Shin and S. Shim, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01576C

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