Jump to main content
Jump to site search


Prophylactic role of Vitamin K supplementation on vascular inflammation in type 2 diabetes by regulating NF-κB/Nrf2 pathway via activating Gla proteins

Abstract

There is no previous study that has examined the relationship between circulating vitamin K1 (VK1) with vascular inflammation in type 2 diabetes (T2D). This study aims to examine the hypothesis that circulating VK1 deficiency may be associated with higher inflammation and insulin resistance in T2D patients and that VK1 supplementation regulates NF-κB/Nrf2 pathway via activating VK-dependent Gla proteins and reduces vascular inflammation. Results showed that plasma VK1 levels were significantly lower and MCP-1, fasting glucose, HbA1c, and insulin resistance (HOMA-IR) were significantly higher in T2D patients compared to controls. Lower levels of VK1 in T2D patients were significantly and inversely correlated with MCP-1 and HOMA-IR, which suggests that VK1 supplementation may reduce the vascular inflammation and insulin resistance in T2D. Using high fat diet-fed T2D mice model this study further demonstrated that VK1 supplementation (1, 3, 5 µg/kg BW, 8 weeks) dose-dependently decreased the body weight gain, glucose intolerance, fasting glucose, glycated hemoglobin, HOMA-IR, and cytokine secretion (MCP-1 and IL-6) in T2D mice. Further cell culture studies showed that VK1 supplementation (1, 5, or 10 nM) decreased the NF-κB phosphorylation and MCP-1 secretion and increased the Nrf2 protein expression in high glucose (HG, 25 mM)-treated monocytes. Signal silencing studies with GGCX siRNA again depicted the role of VK-dependent Gla proteins in mediating the effect of VK1 on vascular inflammation in HG-treated cells. In conclusion, this study suggests that circulating VK1 has a positive effect on lowering vascular inflammation in T2D by regulating NF-κB/Nrf2 transcription factors via activating VK-dependent Gla proteins.

Back to tab navigation

Publication details

The article was received on 26 Sep 2017, accepted on 26 Nov 2017 and first published on 27 Nov 2017


Article type: Paper
DOI: 10.1039/C7FO01491K
Citation: Food Funct., 2017, Accepted Manuscript
  •   Request permissions

    Prophylactic role of Vitamin K supplementation on vascular inflammation in type 2 diabetes by regulating NF-κB/Nrf2 pathway via activating Gla proteins

    A. Dihingia, D. Ozah, P. K. Baruah, J. Kalita and P. Manna, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01491K

Search articles by author

Spotlight

Advertisements