Jump to main content
Jump to site search


Structure characterization of a novel polysaccharide from Hericium erinaceus fruiting bodies and its immunomodulatory activities

Abstract

A novel polysaccharide fraction (HEP-S) was extracted and isolated from the fruiting bodies of Hericium erinaceus. Structural characterization revealed that HEP-S had an average molecular weight of 1.83×104 Da and consisted of rhamnose, fucose, mannose, glucose and galactose at molar ratio of 1.47:0.93:1.36:8.68:4.08. Periodate oxidation-Smith degradation and NMR analysis showed that the main linkage types of HEP-S were composed of (1→)-α-D-Glc, (1→3,4)-α-D-Glc, (1→6)-α-D-Gal, (1→3,4)-β-D-Man, (1→3,6)-α-Rha and (1→2)-β-L-Fuc. The immunomodulatory assay indicated that HEP-S could significantly enhance the pinocytic and phagocytic capacity and promote the nitric oxide and pro-inflammatory cytokines secretion through activating corresponding mRNA and protein expression in RAW 264.7 cells involving toll-like receptor 2 membrane receptor. Besides, HEP-S was also found to improve the adaptive immune function through enhancing T and B lymphocyte proliferation and increasing the interleukin-2, interleukin-4 and interferon-γ secretion in spleen lymphocyte. These results suggested that HEP-S could be used as a potential immunoregulatory agent in functional foods.

Back to tab navigation

Publication details

The article was received on 07 Sep 2017, accepted on 06 Nov 2017 and first published on 09 Nov 2017


Article type: Paper
DOI: 10.1039/C7FO01389B
Citation: Food Funct., 2017, Accepted Manuscript
  •   Request permissions

    Structure characterization of a novel polysaccharide from Hericium erinaceus fruiting bodies and its immunomodulatory activities

    F. Wu, C. Zhou, D. Zhou, S. Ou, X. Zhang and H. Huang, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01389B

Search articles by author

Spotlight

Advertisements