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Characterization of fecal fat composition and gut derived fecal microbiota in high-fat diet fed rats following intervention with chito-oligosaccharide and resistant starch complexes

Abstract

The current study analyzed the different effects of intervention in high-fat diet fed rats using chito-oligosaccharides (CO group), resistant starch (RS group) and their complexes (CO-RS group), respectively. Properties such as fecal fat composition, gut derived fecal microbiota and fecal metabolites were investigated. The results show that supplementation with CO-RS in the high-fat diet led to the highest level of fecal fat excretion, followed by the CO and RS groups, and its fatty acid composition was characterized by a lower ∑UFA and higher ∑SFA levels. Furthermore, CO-RS consumption significantly enhanced the excretion of bile acids in the feces, which might be associated with a higher conversion of cholesterol into bile acids and the enhanced biding capacity with the bile acids. Fecal microbiota profile using amplified V4 rDNA suggested that rats in CO-RS group developed an increased richness and diversity in the gut bacterial community compared to the high-fat diet group. More importantly, CO-RS intake significantly increased the abundance of both Lactobacillus and Bifidobacterium, indicating their potential use as prebiotics. Furthermore, the highest abundance of Allobaculum and Blautia genus in the feces of CO-RS group was also found in this study, which is highly related to the highest production of short-chain fatty acids (SCFAs) in the colon among all the animals groups. Histological analysis of colon tissue revealed that the CO-RS supplemented diet was associated with a greater degree of thickness of the mucosa layer compared to the rats in the untreated high-fat diet group. The resulting shift in the gut microbiome, increased metabolite (SCFAs) production and increased thickness of the mucosal layer may provide a profound influence of CO-RS consumption on gut protection.

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Publication details

The article was received on 12 Aug 2017, accepted on 23 Sep 2017 and first published on 25 Sep 2017


Article type: Paper
DOI: 10.1039/C7FO01244F
Citation: Food Funct., 2017, Accepted Manuscript
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    Characterization of fecal fat composition and gut derived fecal microbiota in high-fat diet fed rats following intervention with chito-oligosaccharide and resistant starch complexes

    W. Shang, X. Si, Z. Zhou, Y. Li, P. Strappe and C. Blanchard, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01244F

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