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A Potential Strategy for Counteracting Age-Related Sarcopenia: Preliminary Evidence of Combined Exercise Training and Leucine Supplementation

Abstract

Previous research has demonstrated the positive effects of concurrent/combined aerobic and resistance exercise or leucine supplementation on skeletal muscle protein synthesis (MPS) and hypertrophy in aging organisms. However, the effects of a multimodal intervention which combines both aerobic and resistance exercise and leucine supplementation has not been fully elucidated. Eighteen month old and 2-month-old C57BL/6 mice were assigned to aging control (AC, n=8), aging and multimodal intervention (AMI, n=8) and young control (YC, n=8). Mice in YC and AC group were fed an alanine-rich diet (3.4%), mice in AMI group received the isonitrogenous leucine-supplemented (5%) diet in combination with combined aerobic (30 minutes swimming) and resistance exercise training (incremental jumping submersed in water with overload corresponding to 40%-50% body weight) for a total of 4 weeks. The gastrocnemius muscles were dissected for western blotting detection (signaling proteins involved in MPS) and the ex vivo determination of protein synthesis and protein content. The muscle strength of the hind limbs were measured pre-experiment and repeated once per week on Sunday for 4 weeks. Mice in AC and AMI group showed lower ex vivo protein synthesis, protein content, expression of signaling proteins involved in MPS, maximal grip strength but higher plasma cortisol compared with YC post intervention. When compared to AC mice, the multimodal treatment led to lower activity of Sestrin2, higher expression of PI3K Ⅲ and the phosphorylation of mTOR, p70S6K and 4E-BP1, as well as higher plasma leucine, wet gastrocnemius muscle weight and muscle weight to body weight ratio. Furthermore, the multimodal intervention induced more pronounced anabolic response such as higher ex vivo protein synthesis rate, total protein content, and myofibrillar fractions in gastrocnemius muscle, and greater maximum grip strength. The present research shows that a multimodal intervention including combined both aerobic and resistance exercise training and 5% leucine supplementation has potential to maintain skeletal muscle protein synthesis and attenuate losses in muscular strength during the aging process.

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Publication details

The article was received on 01 Aug 2017, accepted on 11 Oct 2017 and first published on 12 Oct 2017


Article type: Paper
DOI: 10.1039/C7FO01181D
Citation: Food Funct., 2017, Accepted Manuscript
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    A Potential Strategy for Counteracting Age-Related Sarcopenia: Preliminary Evidence of Combined Exercise Training and Leucine Supplementation

    Z. XIA, J. Cholewa, Y. Zhao, Y. Yang, H. Shang, H. Jiang, Q. Su and N. E. Zanchi, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO01181D

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