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Reshaped fecal gut microbiota composition by the intake of high molecular weight persimmon tannin in normal and high-cholesterol diet-fed rats

Abstract

It has been proposed that the gut microbiome may be related to obesity, and diet-induced obesity may induce changes in the normal gut microbiota compositions. Our previous studies suggested that persimmon tannin (PT), which is highly polymerized and nonabsorbable in the intestine, showed anti-hyperlipidemia and cholesterol-lowering effects in animal models. Considering possible composition modifications in intestinal bacteria might contribute to its anti-hyperlipidemia and cholesterol-lowering effects in vivo, in the present study, we determined whether PT administration could modify the gut microbiota in normal diet and high-cholesterol (HC) diet rats, and how PT alter the bacterial compositions in both normal and HC feeding rats. Sprague-Dawley rats were randomized into eight groups and fed a normal or HC diet supplemented with or without low/medium/high dose of PT (50 (LPT), 100 (MPT), 200 (HPT) mg/kg•body weight (BW)/day, respectively) for 4 weeks. At the day 0, 7, 14, 21 and 28, faeces were collected and prepared for the detection of microbiota and physicochemical analysis. The results showed that LPT and MPT supplementation significantly altered gut microbiota composition by increasing Bacteroidetes/Proteobacteria ratio both in normal rats and HC-diet-fed rats. LPT also decreased Firmicutes/Bacteroidetes ratio in normal diet rats and MPT decreased Firmicutes/Bacteroidetes ratio in HC diet rats. Both LPT and MPT supplementation generated a significant alteration in specific bacterial species after 14 days treatment. They increased Bifidobacterium sp., Lactobacillus sp., and reduced the relative abundance of Escherichia coli and Enterococcus. Our data also indicated that there was a correlation between changes in bacterial composition and change in fatty acid (SCFAs) metabolism. However, HPT supplementation altered the gut microbiota in phylum and species levels in an adverse way.

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Publication details

The article was received on 05 Jul 2017, accepted on 17 Nov 2017 and first published on 28 Nov 2017


Article type: Paper
DOI: 10.1039/C7FO00995J
Citation: Food Funct., 2017, Accepted Manuscript
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    Reshaped fecal gut microbiota composition by the intake of high molecular weight persimmon tannin in normal and high-cholesterol diet-fed rats

    W. Zhu, K. Lin, K. Li, X. Deng and C. Li, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO00995J

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