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Ginseng (Panax ginseng Meyer) oligopeptides regulate innate and adaptive immune responses in mice via increased macrophage phagocytosis capacity, NK cell activity and Th cells secretion

Abstract

Ginseng (Panax ginseng Meyer), traditionally used as a restorative medicine, has been the most widely used and acclaimed herb in Chinese communities for thousands of years. To investigate immune-modulating activity of ginseng oligopeptides (GOP), 420 healthy female BALB/c mice were intragastrically administered distilled water (control), whey protein (0.15 g/kg body weight, BW), GOP 0.0375, 0.075, 0.15, 0.3 and 0.6 g/kg BW for 30 days. Blood samples of the mice were collected from ophthalmic venous plexus and then sacrificed by cervical dislocation. Four experiments were conducted to determine immunomodulatory effects of GOP on innate and adaptive immune responses. Then flow cytometry was conducted to investigate spleen T lymphocyte subpopulations, multiplex sandwich immunoassays to investigate serum cytokine and immunoglobulin levels and ELISA to investigate intestinal secreted immunoglobulin to study the mechanism of GOP affecting immune system. We confirmed that GOP enhanced innate and adaptive immune responses in mice, which was attributed to improving cell-mediated and humoral immunity, macrophage phagocytosis capacity and NK cell activity. Of note, the effects of GOP on the above functions were better than whey protein, revealing immune-modulating activity should be due to GOP, but not protein. From multiple assay results, we found that the immune-modulating activity might be due to the increased macrophage phagocytosis capacity and NK cell activity, and the enhancement of T and Th cells, following with IL-2, IL-6 and IL-12 secretion and IgA, IgG1 and IgG2b production. GOP could be a candidate functional food to improve immune function, and the appropriate level was 0.3 to 0.6 g/kg BW.

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Publication details

The article was received on 27 Jun 2017, accepted on 10 Aug 2017 and first published on 11 Aug 2017


Article type: Paper
DOI: 10.1039/C7FO00957G
Citation: Food Funct., 2017, Accepted Manuscript
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    Ginseng (Panax ginseng Meyer) oligopeptides regulate innate and adaptive immune responses in mice via increased macrophage phagocytosis capacity, NK cell activity and Th cells secretion

    L. He, J. Ren , R. Liu , Q. Chen, J. Zhao, X. Wu, Z. Zhang, J. Wang, G. Pettinato and Y. Li, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO00957G

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