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Issue 9, 2017
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Rapid identification of α-glucosidase inhibitors from Dioscorea opposita Thunb peel extract by enzyme functionalized Fe3O4 magnetic nanoparticles coupled with HPLC-MS/MS

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Abstract

Dioscorea opposita Thunb, commonly known as “yam” that has a long dietary therapy history for diabetes, is widely consumed as a botanical dietary supplement and widely cultivated in China. In this work, a method for rapid screening of α-glucosidase inhibitors from Dioscorea opposita Thunb peel extract was developed using α-glucosidase functionalized magnetic nanoparticles (αG-MNPs) as a solid phase extraction absorbent in combination with high performance liquid chromatography-mass spectrometry (HPLC-MS). Two α-glucosidase inhibitors were selectively extracted and identified as batatasin I and 2,4-dimethoxy-6,7-dihydroxyphenanthrene. Their α-glucosidase inhibitory activities (IC50 = 2.55 mM and 0.40 mM, respectively) were significantly higher than that of acarbose (as control). Taking advantage of the specificity in enzyme binding and the convenience of magnetic separation, this method has great potential for rapid and fast screening of α-glucosidase inhibitors from complex natural resources.

Graphical abstract: Rapid identification of α-glucosidase inhibitors from Dioscorea opposita Thunb peel extract by enzyme functionalized Fe3O4 magnetic nanoparticles coupled with HPLC-MS/MS

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Publication details

The article was received on 23 Jun 2017, accepted on 25 Jul 2017 and first published on 26 Jul 2017


Article type: Paper
DOI: 10.1039/C7FO00928C
Citation: Food Funct., 2017,8, 3219-3227
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    Rapid identification of α-glucosidase inhibitors from Dioscorea opposita Thunb peel extract by enzyme functionalized Fe3O4 magnetic nanoparticles coupled with HPLC-MS/MS

    S. Zhang, D. Wu, H. Li, J. Zhu, W. Hu, M. Lu and X. Liu, Food Funct., 2017, 8, 3219
    DOI: 10.1039/C7FO00928C

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