Jump to main content
Jump to site search


In vitro and in vivo inhibitory effects of a Pleurotus eryngii protein on colon cancer cells

Author affiliations

Abstract

Edible mushrooms have been considered as a good source of bioactive components with various health benefits. Our previous study demonstrated the identity of a novel protein from Pleurotus eryngii, namely PEP, and its anti-inflammatory activities. Herein, we further determined the inhibitory effects of PEP against colon cancer cells. PEP suppressed the proliferation of human and murine colon cancer HCT116 and MC38 cells in a dose and time-dependent fashion, while it showed no inhibitory effect on normal human colonic myofibroblasts CCD-18Co at the same concentrations tested. Moreover, PEP induced cell cycle arrest and led to extensive cellular apoptosis in colon cancer cells, which was associated with the downregulation of cell cycle-related signaling proteins, e.g. cyclin B, cyclin E and cdc-2, and the upregulation of apoptosis-related signaling proteins, e.g. p53 and c-PARP. The results from an in vivo study showed that PEP treatment significantly suppressed tumor development of allograft colon cancer cells in mice, and this inhibition was associated with the upregulation of p21, p53, caspase-3 and cleaved caspase-3. Overall, our results provided a basis for PEP as a promising preventive agent against colon cancer.

Graphical abstract: In vitro and in vivo inhibitory effects of a Pleurotus eryngii protein on colon cancer cells

Back to tab navigation

Publication details

The article was received on 17 Jun 2017, accepted on 14 Aug 2017 and first published on 18 Aug 2017


Article type: Paper
DOI: 10.1039/C7FO00895C
Citation: Food Funct., 2017, Advance Article
  •   Request permissions

    In vitro and in vivo inhibitory effects of a Pleurotus eryngii protein on colon cancer cells

    B. Yuan, N. Ma, L. Zhao, E. Zhao, Z. Gao, W. Wang, M. Song, G. Zhang, Q. Hu and H. Xiao, Food Funct., 2017, Advance Article , DOI: 10.1039/C7FO00895C

Search articles by author

Spotlight

Advertisements