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Averrhoa carambola free phenolic extract ameliorates nonalcoholic hepatic steatosis by modulating mircoRNA-34a, mircoRNA-33 and AMPK pathway in leptin receptor-deficient db/db mice

Abstract

The objective of the present study is to investigate the hepatic steatosis relieving effect of Averrhoa carambola free phenolic extract(ACF)on leptin receptor-deficient (db/db) mice, and elucidate the modulation hepatic lipogenesis mechanisms. Serum Alanine aminotransferase (ALT) and Aspartate aminotransferase (AST) assay, accompanied hematoxylin and eosin (H&E) staining were applied to identify the alleviation of the liver histopathological changes. Serum and hepatic lipids assay, combined with Oil Red O staining were used to investigate the amelioration of the lipid accumulation. Further assessments by quantitative real-time PCR and Western blot assays were used to elucidate the suppression of fatty acid and triglyceride (TG) synthesis mechanisms underlying ACF protection. These results indicated that ACF treatment significantly reduced the liver TG of db/db mice (p<0.05). The mechanisms are partly through phosphorylation of AMPK α and down-regulation the expression of SREBP-1c, further down-regulation of FAS and SCD1 (p<0.05). In addition, the expression levels of mircoRNA-34a and mircoRNA-33 which directly modulate this signaling pathway were significantly down-regulated by ACF treatment (p<0.05). Collectively, these results revealed that ACF exhibited potent hepatic steatosis relieving effect partly by inhibiting the signal transduction of hepatic lipogenesis.

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Publication details

The article was received on 08 Jun 2017, accepted on 02 Oct 2017 and first published on 11 Oct 2017


Article type: Paper
DOI: 10.1039/C7FO00833C
Citation: Food Funct., 2017, Accepted Manuscript
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    Averrhoa carambola free phenolic extract ameliorates nonalcoholic hepatic steatosis by modulating mircoRNA-34a, mircoRNA-33 and AMPK pathway in leptin receptor-deficient db/db mice

    D. Pang, L. You, L. Zhou, T. Li, B. Zheng and R.H. Liu, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO00833C

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