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Chlorogenic acid protects against aluminium-induced cytotoxicity through chelation and antioxidant actions in primary hippocampal neuronal cells

Abstract

Chlorogenic acid (CGA), a major polyphenolic component of many plants, displays antioxidant and neuroprotective properties in neurodegenerative diseases. To investigate whether CGA may influence aluminium (Al) induced cytotoxicity, aluminium chloride (50 μM Al) was administered in primary hippocampal neuronal cells presupplemented with CGA (10, 50 and 100 μM). Our study shows that the exposure to Al caused cell death, Al3+ accumulation, reactive oxygen species generation and mitochondrial damage in cells. Administration of CGA (50 μM) increased cell viability by 37.5%, decreased the levels of Al3+ by 26.0%, together with significantly weakened the oxidative damage compared with Al treatment alone. CGA protected neurons against Al-induced oxidative stress by increasing the expression of nuclear factor-E2-related factor 2 (Nrf2) and its target phase 2 enzymes. Administration of CGA remarkably promoted the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, creatine kinase and acetylcholinesterase and attenuated the rate of ATP hydrolysis. Our finding shows that CGA has neuroprotective effects against Al-induced cytotoxicity by the chelation and antioxidant activation.

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Publication details

The article was received on 03 May 2017, accepted on 05 Jul 2017 and first published on 07 Jul 2017


Article type: Paper
DOI: 10.1039/C7FO00659D
Citation: Food Funct., 2017, Accepted Manuscript
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    Chlorogenic acid protects against aluminium-induced cytotoxicity through chelation and antioxidant actions in primary hippocampal neuronal cells

    X. Wang, X. Fan, S. Yuan, W. Jiao, B. Liu, J. Cao and W. Jiang, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO00659D

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