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Anti-tumor bioactivities of curcumin on mice loaded with gastric carcinoma

Abstract

Curcumin, a kind of derivative from dried rhizome of curcuma longa, has proved to possess anti-tumor effects. However, the detailed molecular mechanisms have not been fully elucidated. In this study we aimed to explore the anti-tumor mechanisms of curcumin in treating gastric cancer. BALB/C mice grafted with a mouse gastric adenocarcinoma cell line MFC were used as the experimental model. Mice received different doses of curcumin after grafting. Tumor size was measured and tumor weight was determined after tumor inoculation. TUNNEL assay and flow cytometric analysis were applied to evaluate the apoptosis of cancer cells. Serum cytokines IFN-γ, TNF-α, granzyme B and perforin were detected by ELISA assay. The anti-tumor effect was determined by the cytotoxic T-lymphocyte (CTL) assays and in vivo tumor prevention test. The expressions of DEC1, HIF-1α, STAT3 and VEGF in tumor tissues were examined by immunostaining and analyzed by Image J analysis system. Compared with controls, tumor growth (size and weight) was significantly inhibited by curcumin treatment (P < 0.05). Apoptotic index in gastric cancer cells was significantly increased in curcumin treatment group. Splenocytes cells from mice treated with curcumin exhibited higher cytolytic effects on MFC cancer cells than those from mice treated with saline (P < 0.01). The expressions of DEC1, HIF-1α, STAT3 and VEGF in tumor tissues were down-regulated after curcumin treatment. Our results indicated that curcumin inhibited the proliferation of gastric carcinoma by inducing the apoptosis of tumor cells, activating the effects of immune cells to secret a large amount of cytokines, and down-regulating DEC1, HIF-1α, VEGF and STAT3 signal transduction pathways.

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Publication details

The article was received on 11 Apr 2017, accepted on 07 Aug 2017 and first published on 09 Aug 2017


Article type: Paper
DOI: 10.1039/C7FO00555E
Citation: Food Funct., 2017, Accepted Manuscript
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    Anti-tumor bioactivities of curcumin on mice loaded with gastric carcinoma

    X. Wang, Q. Wang, H. Lin and N. Chang, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C7FO00555E

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