Jump to main content
Jump to site search

Issue 8, 2017
Previous Article Next Article

Oleuropein represses the radiation resistance of ovarian cancer by inhibiting hypoxia and microRNA-299-targetted heparanase expression

Author affiliations

Abstract

Radiotherapy in ovarian cancer frequently invokes resistance; this severely compromises its therapeutic effect and results in poor clinical prognosis. How to overcome the acquired resistance and re-sensitize tumors to radiation is the central question in this clinical setting. Cancer cell survival was evaluated using a clonogenic assay. The microRNA expression profile was analyzed using a microarray. Transcript expression was determined using real time PCR. The expression of protein was determined by immunoblotting. Transcription activation was measured using a luciferase reporter assay. Transcription factor binding was determined using ChIP-PCR. Xenograft model was established and exposed to radiation with the simultaneous administration of oleuropein. Tumor growth was monitored. We demonstrated that treatment of oleuropein-sensitized ovarian cells with radiation altered the microRNA expression profile. The endogenous expression of miR-299 was suppressed by a hypoxia inducible factor and relieved in response to oleuropein, which in turn suppressed HPSE1 expression and consequently led to increased sensitivity to radiation. Our data elucidates an unappreciated mechanism mediating radiotherapy resistance in ovarian cancer and exploits the potential synergistic effect of oleuropein with radiation, which warrants further clinical investigation.

Graphical abstract: Oleuropein represses the radiation resistance of ovarian cancer by inhibiting hypoxia and microRNA-299-targetted heparanase expression

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Apr 2017, accepted on 01 Jul 2017 and first published on 20 Jul 2017


Article type: Paper
DOI: 10.1039/C7FO00552K
Citation: Food Funct., 2017,8, 2857-2864
  •   Request permissions

    Oleuropein represses the radiation resistance of ovarian cancer by inhibiting hypoxia and microRNA-299-targetted heparanase expression

    Y. Xing, D. Cui, S. Wang, P. Wang, X. Xing and H. Li, Food Funct., 2017, 8, 2857
    DOI: 10.1039/C7FO00552K

Search articles by author

Spotlight

Advertisements