Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae
Pleurotus ferulae is an edible mushroom and has been used in Uygur medicine for a long history. In this study, we purified the polysaccharides from P. ferulae (PFPS) and investigated its structural characteristics. We obtained the homogenous PFPS with around 1600 kD of molecular weight and prominent characteristic polysaccharide groups, which mainly contained glucose (97%), followed by mannose and galactose (3%). Both 1H and 13C NMR spectra indicated that PFPS contained both α- and β-anomeric configurations. Atomic force microscopy and Congo red-staining data further suggested that PFPS was belonged to linear branched structure that existed in flexible single chains at low concentration and could form aggregates such as triple-helical structure at high concentration. Moreover, PFPS promoted maturation of dendritic cells through TLR4 mediated signaling pathway, which characterized by the increased expressions of CD40, CD86, IL-12 and TNF-α and the decreased endocytosis. The results suggest that PFPS have the immunoregulatory activity.