Jump to main content
Jump to site search

Issue 9, 2017
Previous Article Next Article

Profiles of free and bound phenolics extracted from Citrus fruits and their roles in biological systems: content, and antioxidant, anti-diabetic and anti-hypertensive properties

Author affiliations

Abstract

This study of selected plants of the Rutaceae family was carried out to investigate their phenolic content, antioxidant activity, and the in vitro inhibitory potential of extracted phenolics towards enzymes relevant for hyperglycemia and hypertension. The phenolic content, antioxidant activity and phenolic extract-mediated inhibitory activities for α-glucosidase and α-amylase were evaluated by spectrophotometry. The content of individual phenolics and the angiotensin I-converting enzyme (ACE) inhibitory activity of the phenolic extracts were evaluated by LC/MS-MS and RP-HPLC methods, respectively. A higher percentage of free phenolic content was seen for all the selected plants of the Rutaceae family (85.43–92.82% of the total phenolic content) than of the bound form (7.18–14.57% of total phenolic content). The major predominant bound phenolic in lemon and red blood orange was hesperidin. The major predominant bound phenolic in pummelo, shamouti and clementine was ferulic acid. The highest ACE and α-glucosidase inhibitory activity of the extracted phenolics from lemon was associated with free phenolic extracts obtained at 30 °C with values of 100% inhibition. Red blood orange free phenolic extract (30 °C) elicited the highest α-amylase inhibition activity (32.3%). In contrast, extracted bound phenolics after acid and base hydrolysis from all selected plants from the Citrus species were shown to induce activation of the ACE and α-amylase enzymes.

Graphical abstract: Profiles of free and bound phenolics extracted from Citrus fruits and their roles in biological systems: content, and antioxidant, anti-diabetic and anti-hypertensive properties

Back to tab navigation

Publication details

The article was received on 09 Feb 2017, accepted on 12 Jul 2017 and first published on 14 Jul 2017


Article type: Paper
DOI: 10.1039/C7FO00212B
Citation: Food Funct., 2017,8, 3187-3197
  •   Request permissions

    Profiles of free and bound phenolics extracted from Citrus fruits and their roles in biological systems: content, and antioxidant, anti-diabetic and anti-hypertensive properties

    M. H. Alu'datt, T. Rababah, M. N. Alhamad, M. A. Al-Mahasneh, K. Ereifej, G. Al-Karaki, M. Al-Duais, J. E. Andrade, C. C. Tranchant, S. Kubow and K. A. Ghozlan, Food Funct., 2017, 8, 3187
    DOI: 10.1039/C7FO00212B

Search articles by author

Spotlight

Advertisements