Ameliorative effects of pepsin-digested chicken liver hydrolysates on development of alcoholic fatty livers in mice
As the development of economics and increasing working loadings, the alcohol abuse becomes more and more severe, and thus, leading occurrences of alcoholic liver disease (ALD). Pepsin-digested chicken liver hydrolysates (CLHs) contain high amounts of glutamic acid, leucine, lysine, and alanine while the contents of taurine, anserine, and carnosine were also elevated after a pepsin hydrolyzation. The objectives of this study were to evaluate the protective effects of CLHs against chronic alcohol consumption. The results indicated that the enlarged (p < 0.05) sizes of liver and spleen, and serum AST, ALT, and ALKP levels of alcoholic diet fed mice were ameliorated by supplementing CLHs. Moreover, increased hepatic immunocyte infiltration shown on the H&E staining and higher (p < 0.05) hepatic triglyceride contents, TBARS values, and proinflammatory cytokine levels in alcoholic diet fed mice were reduced (p < 0.05) by supplementing CLHs as well. Those benefits were attributed to up-regulated fatty acid β-oxidation and down-regulated fatty acid synthesis, as well as increased (p < 0.05) SOD, CAT, and GPx activities, TEAC levels, as well as elevated alcohol metabolic enzymatic activities (ALDH).