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Protective Effects of Fraction 4a of Polysaccharides Isolated from Lycium barbarum Against KBrO3-induced Renal Damage in Rats

Abstract

Potassium bromate (KBrO3) is widely used as a food additive and is a major water disinfection by-product. The aim of this study is to evaluate the protective effects of fraction 4a of polysaccharides isolated from Lycium barbarum (LBP-4a) on KBrO3-induced renal damage in rats, and explore the relevant mechanisms behind it. Male Wistar rats were divided into normal control group, KBrO3 control group, LBP-4a(L) and LBP-4a(H) groups. Except normal control group, the rats in other three groups were administrated with KBrO3 by intraperitoneal injection to induce renal damage. Before renal damage induction, LBP-4a(L) and LBP-4a(H) group mice were pretreated with 50 mg/kg b.w or 100mg/kg b.w by intragatric administration. Clinical biochemistry parameters and markers of oxidative damage were determined further. Treatment with LBP-4a administration prior to KBrO3 challenge prevented an increase in the levels of nitrite, creatinine, urea nitrogen and uric acid levels in serum, increased the activities of antioxidant enzymes in kidney tissues. Comet assay and FCS results showed that pretreatment with LBP-4a also alleviated DNA damages and decreased the mitochondrial membrane potentials in renal cells. Histological studies further supported these results of above, showing extensive renal damage in KBrO3-treated animals and greatly reduced tissue injury in LBP-4a pretreatment groups. In conclusion, LBP-4a exhibited protective effects on KBrO3-induced renal damage, and the mechanism was closely correlated with a reduction in lipid peroxidation levels and an increase in antioxidant enzyme activities in kidney tissues, the alleviating DNA damage and increasing mitochondrial membrane potential in renal cells. These observations provided the background for the further development of LBP-4a as a type of protective agent used in renal damage treatment.

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Publication details

The article was received on 19 Dec 2016, accepted on 14 Jun 2017 and first published on 14 Jun 2017


Article type: Paper
DOI: 10.1039/C6FO01818A
Citation: Food Funct., 2017, Accepted Manuscript
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    Protective Effects of Fraction 4a of Polysaccharides Isolated from Lycium barbarum Against KBrO3-induced Renal Damage in Rats

    J. Li, M. Shi, B. Ma, Y. Zheng, R. Niu and K. Li, Food Funct., 2017, Accepted Manuscript , DOI: 10.1039/C6FO01818A

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