Identification of two novel antioxidant peptides from edible bird’s nest (Aerodramus fuciphagus) protein hydrolysate
Edible bird’s nest (EBN) is widely consumed as a precious food and traditional medicine amongst Chinese. In present study, for the first time, the antioxidant properties of EBN pepsin-trypsin hydrolysate of swiftlet species Aerodramus fuciphagus and its ultrafiltration fractions were investigated. Thirteen peptides with molecular weights between 514.29 to 954.52 Da were identified in EBN fraction with the aim of mass spectrometry. Two novel pentapeptides Pro-Phe-His-Pro-Tyr and Leu-Leu-Gly-Asp-Pro, corresponding to f134-138 and f164-168 of cytochrome b of A.fuciphagus indicated the highest ORAC values of 14.95 and 14.32 µM of TE µM-1 peptide, respectively. Both purified peptides showed resistance against simulated gastrointestinal proteases. In addition, both peptides had no in vitro cytotoxicity in human lung MRC-5 cells and prevented human liver carcinoma HepG2 cellular damage caused by hydroxyl radicals. Therefore, it is suggested that EBN protein hydrolysates are good source of natural antioxidants and could be applied as nutraceutical compounds.