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Issue 3, 2017
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Sialoglycoprotein isolated from the eggs of Gadus morhua enhances fracture healing in osteoporotic mice

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Abstract

Osteoporosis is a common disease in the elderly, which is related to fracture healing delay. In this study, the effects of treatment with sialoglycoprotein isolated from the eggs of Gadus morhua (Gm-SGP) on tibial fracture healing in ovariectomized (OVX) osteoporotic female C57BL/6J mice for 56 days post-fracture were investigated. The result showed that Gm-SGP treatment significantly increased serum angiogenic factors and bone formation markers on day 5 and 11 post-fracture when compared with the OVX group. In addition, histological results in the Gm-SGP group showed a stronger endochondral ossification, a stronger bony consolidation and a stronger bony callus remodeling capability on day 11, 24 and 35 post-fracture, respectively, in comparison with the OVX group. Meanwhile, micro-computerized tomography revealed that the Gm-SGP group had stronger bony callus remodeling capability as evidenced by higher BV/TV and Tb.N but lower Tb.Sp and shorter lengths of callus maximum cross section than the OVX group on day 24 post-fracture. Besides, the tibial callus bending stiffness was significantly enhanced in the Gm-SGP group as compared with the OVX group on day 56 post-fracture. Moreover, gene expression suggested that Gm-SGP promoted vascular invasion and endochondral ossification on day 11 post-fracture as well as bone formation on day 11 and 24 post-fracture via up-regulating the expression of angiogenesis factors (including VEGF, PDGF and Ang1), entochondrostosis factors (including Col2a1, Aggrecan, Col10a1 and MMP-13) and osteogenesis markers (including Col1a1, BMP-2 and OCN). This research suggests that Gm-SGP significantly improve fracture healing which is delayed by OVX-induced osteoporosis. The present study may contribute to providing important implications for the utilization of Gm-SGP from fish eggs as a functional food to enhance fracture healing.

Graphical abstract: Sialoglycoprotein isolated from the eggs of Gadus morhua enhances fracture healing in osteoporotic mice

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Publication details

The article was received on 12 Sep 2016, accepted on 17 Jan 2017 and first published on 18 Jan 2017


Article type: Paper
DOI: 10.1039/C6FO01346E
Citation: Food Funct., 2017,8, 1094-1104
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    Sialoglycoprotein isolated from the eggs of Gadus morhua enhances fracture healing in osteoporotic mice

    Q. Zhan, X. Gui, F. Wang, P. Yu, M. Zhao, J. Wang and C. Xue, Food Funct., 2017, 8, 1094
    DOI: 10.1039/C6FO01346E

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