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Chiral self-assembly of fullerene clusters on CT-DNA templates

Abstract

Herein we discuss the differential interaction of three monosubstituted fullerene derivatives having pyridinium, aniline or phenothiazine end groups (F-Py, F-An and F-PTz, respectively) with calf thymus DNA (CT-DNA) probed via spectroscopic and imaging techniques. The pyridinium derivative, F-Py gets molecularly dissolved in 10% DMSO-PBS and interact with CT-DNA via groove binding and electrostatic interactions leading to initial condensation of CT-DNA into micrometer sized aggregates and subsequent precipitation. On the other hand, the aniline derivative F-An, which is reported to form nanoclusters of 3-5 nm size, interact with DNA through ordered, chiral assemblies on CT-DNA template perturbing the highly networked structure of CT-DNA to form nanonetworks, which eventually transform to condensed aggregates. The binding interactions between CT-DNA and F-An nanoclusters were established via UV-Vis, AFM and TEM analysis and the chiral nature of the fullerene nanocluster assemblies on CT-DNA was confirmed through the induced circular dichroism exhibited around 250 nm - 370 nm region, corresponding to the F-An nanocluster absorption. In contrast, the phenothiazine derivative, F-PTz, which forms larger nanoclusters of ~70 nm in 10% DMSO-PBS showed only weak interactions with CT-DNA without affecting its network structure. These results demonstrate the role of the hydrophobic-hydrophilic balance in the design of DNA interacting fullerene derivatives in controlling their cluster size and interactions with CT-DNA and have significance in applications such as DNA condensation, gene delivery and dimension controlled nanomaterial fabrication.

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Publication details

The article was received on 18 Sep 2017, accepted on 05 Oct 2017 and first published on 05 Oct 2017


Article type: Paper
DOI: 10.1039/C7FD00196G
Citation: Faraday Discuss., 2017, Accepted Manuscript
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    Chiral self-assembly of fullerene clusters on CT-DNA templates

    S. K. Vittala and J. Joseph, Faraday Discuss., 2017, Accepted Manuscript , DOI: 10.1039/C7FD00196G

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