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Volume 207, 2018
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Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

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Abstract

Fluorescent proteins exhibit interesting excited state photochemistry, leading to bright fluorescence emission that renders their versatile biological role and wide use as biomarkers. A molecular-level mechanism of the excited state dynamics is desirable to pinpoint the origin of the bright fluorescence of these proteins. Here we present studies on a yellow fluorescent protein variant, Venus, and investigate the photophysics behind the dual fluorescence emission upon UV excitation. Based on our studies, we propose that the unique nature of the potential energy surface is responsible for the observation of minor fluorescence in Venus which is not seen in wild type GFP.

Graphical abstract: Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

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Publication details

The article was received on 04 Oct 2017, accepted on 06 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7FD00187H
Citation: Faraday Discuss., 2018,207, 39-54
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    Probing the excited state dynamics of Venus: origin of dual-emission in fluorescent proteins

    S. Dhamija, B. Thakur, P. Guptasarma and A. K. De, Faraday Discuss., 2018, 207, 39
    DOI: 10.1039/C7FD00187H

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