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Probing excited state dynamics of Venus: Origin of dual-emission in fluorescent proteins

Abstract

Fluorescent proteins exhibit interesting excited state photochemistry, leading to bright fluorescence emission that facilitates their versatile biological role and wide use as biomarkers. A molecular-level mechanism of excited state dynamics is required for understanding the origin of the bright fluorescence from these proteins. Here we present studies on a yellow fluorescent protein variant, Venus, and investigate the photophysics behind the dual emission upon UV excitation in fluorescent proteins. Based on our studies, we propose that poorly efficient intramolecular excited state proton transfer (ESPT) and/or intramolecular FÖrster resonance energy transfer (FRET) are responsible for the observation of a minor fluorescence in Venus which is not seen in wild type GFP due to efficient ESPT and FRET.

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Publication details

The article was received on 04 Oct 2017, accepted on 06 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7FD00187H
Citation: Faraday Discuss., 2017, Accepted Manuscript
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    Probing excited state dynamics of Venus: Origin of dual-emission in fluorescent proteins

    S. Dhamija, B. Thakur, P. Guptasarma and A. K. De, Faraday Discuss., 2017, Accepted Manuscript , DOI: 10.1039/C7FD00187H

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