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FDSERS17 Dynamic SERS nanosensor for neurotransmitters sensing near neurons

Abstract

Current electrophysiology and electrochemistry techniques have provided unprecedented understanding of neuronal activity. However, these techniques are suited for a small, albeit important, panel of neurotransmitters such as glutamate, GABA and dopamine, but these constitute only a subset of the broader range of neurotransmitters involved in brain chemistry. Surface-enhanced Raman scattering (SERS) provides a unique opportunity to detect a broader range of neurotransmitters in close proximity of neurons. Dynamic SERS (D-SERS) nanosensors based on a patch-clamp-like nanopipettes decorated with gold nanoraspberries can be located accurately under a microscope using techniques analogous to those used in current electrophysiology or electrochemistry experiments. In this paper, we demonstrate that D-SERS can measure in a single experiment ATP, glutamate (glu), acetylcholine (ACh), GABA and dopamine (DA), among other neurotransmitters, with the potential of detecting a greater number of neurotransmitters. The SERS spectra of these neurotransmitters were identified with a barcoding data processing method and time series of the neurotransmitter levels were constructed. The D-SERS nanosensor was then located near dopaminergic neurons. Detection of neurotransmitters was performed in response to a series of K+ depolarisations , and allowed the detection of elevated levels of both ATP and dopamine. Control experiments were also performed near glial cells, showing only very low basal detection events of neurotransmitters. This paper demonstrates the potential of D-SERS to detect neurotransmitter secretion events near living neurons, but also constitutes a strong proof-of-concept for the broad application of SERS to the detection of secretion events by neurons or other cell types to study normal of pathological cell functions.

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Publication details

The article was received on 11 Apr 2017, accepted on 16 May 2017 and first published on 16 May 2017


Article type: Paper
DOI: 10.1039/C7FD00131B
Citation: Faraday Discuss., 2017, Accepted Manuscript
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    FDSERS17 Dynamic SERS nanosensor for neurotransmitters sensing near neurons

    F. Lussier, T. Brule, M. Bourque, C. Ducrot, L. Trudeau and J. Masson, Faraday Discuss., 2017, Accepted Manuscript , DOI: 10.1039/C7FD00131B

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