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Real-time dynamic SERS detection of galectin using glycan-decorated gold nanoparticles

Abstract

We present the application of surface-enhanced Raman scattering (SERS) spectroscopy for the fast, sensitive and higly specific detection of the galectin-9 (Gal-9) protein in binding buffer (mimicking natural condtions). The method involves the use of specifically designed nanotags comprising glycan-decorated gold nanoparticles encoded with 4-mercaptobenzoic acid. At fast time scales Gal-9 can be detected down to 1.2 nM concentration by monitoring the SERS signal of the reporter, driven by aggregation of the functionalized Au NPs tags, induced by Gal-9 recognition. We additionally demonstrate that the, sensitivity and concentration work range of the sensor can be tuned via control of aggregation dynamics and cluster size distribution.

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Publication details

The article was received on 07 Apr 2017, accepted on 04 May 2017 and first published on 04 May 2017


Article type: Paper
DOI: 10.1039/C7FD00123A
Citation: Faraday Discuss., 2017, Accepted Manuscript
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    Real-time dynamic SERS detection of galectin using glycan-decorated gold nanoparticles

    J. Langer, I. García and L. M. Liz-Marzan, Faraday Discuss., 2017, Accepted Manuscript , DOI: 10.1039/C7FD00123A

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