The study of biocatalysts on the base of the glucose oxidase
In this work we studied the possibility of glucose oxidase (GOx) covalent immobilization on modified inorganic support. The series of GOx-based biocatalysts was synthesized by crosslinking of enzyme on the surface of modified silica and alumina. Polyelectrolites layers were used as modifiers for silica and alumina surface. These layers promote tight binding of GOx with the support. The biocatalyst activity and stability were studied in the oxidation reaction of D-glucose to D-gluconic acid. It was found that GOx immobilized on the modified SiO2 using glutardialdehyde as a crosslinking agent was the most active and stable catalytic system showing 85% of gluconic acid yield. The study of the synthesized biocatalyst structure using FTIR spectroscopy showed that the enzyme was covalently crosslinked to the surface of inorganic support modified with chitosane and glutardialdehyde. In the case of SiO2 the quantity of the immobilized enzyme was higher than in the case of Al2O3.