Jump to main content
Jump to site search

Issue 9, 2017
Previous Article Next Article

Enhanced biofilm penetration for microbial control by polyvalent phages conjugated with magnetic colloidal nanoparticle clusters (CNCs)

Author affiliations

Abstract

Biofilms may shelter pathogenic or other problematic microorganisms that are difficult to eradicate due to hindered penetration of antimicrobial chemicals. Here, we demonstrate the potential for efficient bacterial suppression using polyvalent (broad host-range) phages attached to magnetic colloidal nanoparticle clusters (CNCs) that facilitate biofilm penetration under a relatively small magnetic field (660 gauss). The polyvalent phage PEL1 (Podoviridae family) was immobilized onto Fe3O4-based magnetic CNCs that had been coated with chitosan (and thus functionalized with amino groups). This facilitated conjugation with phages via covalent bonding (i.e., amide linkages) and enabled phage loading, which reached (5.2 ± 0.7) × 103 centers of infection per 1 μg of chitosan-coated CNCs (CS-Fe3O4). The plaque formation capability of PEL1–CS-Fe3O4 on Pseudomonas aeruginosa PA01 and Escherichia coli C3000 lawns was significantly higher than that of phages conjugated with similar CNCs that had been functionalized with carboxyl groups (99.1% vs. 3.2% Petri dish area of infection). In newly established biofilms formed from these two species on a glass surface, PEL1–CS-Fe3O4 removed 88.7 ± 2.8% of the biofilm coverage area after 6 h of treatment. Overall, this conjugation approach could extend the application of phages for microbial control by enhancing their delivery to relatively inaccessible locations within biofilms.

Graphical abstract: Enhanced biofilm penetration for microbial control by polyvalent phages conjugated with magnetic colloidal nanoparticle clusters (CNCs)

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 May 2017, accepted on 18 Jul 2017 and first published on 19 Jul 2017


Article type: Paper
DOI: 10.1039/C7EN00414A
Citation: Environ. Sci.: Nano, 2017,4, 1817-1826
  •   Request permissions

    Enhanced biofilm penetration for microbial control by polyvalent phages conjugated with magnetic colloidal nanoparticle clusters (CNCs)

    L. Li, P. Yu, X. Wang, S. Yu, J. Mathieu, H. Yu and P. J. J. Alvarez, Environ. Sci.: Nano, 2017, 4, 1817
    DOI: 10.1039/C7EN00414A

Search articles by author

Spotlight

Advertisements