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Binding of Al(III) to synthetic RNA. Metal-mediated strand aggregation

Abstract

Over the last years, focused interest in aluminum has been heightened by recent studies regarding its health effects. The possible relation with chronic diseases makes it convenient to address more in depth the reactivity of aluminum with biologically relevant molecules. The present work investigates the interaction of aluminum ion with two synthetic RNAs, poly(rA) and poly(rU), through a detailed thermodynamic and kinetic study. Trivalent aluminum ion was kept in solution by complexation with cacodylate anion, even at neutral pH, thus rendering feasible the study with biological molecules. The results obtained with spectrophotometry, circular dichroism, viscometry and thermal stability measurements indicate that aluminium strongly interacts with single and duplex RNA structures. The kinetic experiments point up that, even though cacodylate was required to keep the metal in solution, actually it inhibits the reaction of aluminum with RNA as it converts the metal into an unreactive dimer species. Notably, further interaction occurred under excess of aluminum/cacodylate complex, inducing aggregation of single-stranded RNAs. An analysis of the kinetic data has shown that the modes of aggregation of the two RNAs differ and such a difference can be ascribed to the diverse polynucleotide secondary structures. The observed stabilization of multiple-stranded systems by aluminum can be of interest for the use of this metal in the study of non-canonical nucleic acid structures.

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Publication details

The article was received on 01 Aug 2017, accepted on 12 Nov 2017 and first published on 13 Nov 2017


Article type: Paper
DOI: 10.1039/C7DT02830J
Citation: Dalton Trans., 2017, Accepted Manuscript
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    Binding of Al(III) to synthetic RNA. Metal-mediated strand aggregation

    B. Garcia, M. Lari, T. Biver, N. Busto, H. Lozano, J. M. Leal and F. Secco, Dalton Trans., 2017, Accepted Manuscript , DOI: 10.1039/C7DT02830J

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