Jump to main content
Jump to site search

Issue 28, 2017
Previous Article Next Article

Two-step FRET mediated metal ion induced signalling responses in a probe appended with three fluorophores

Author affiliations

Abstract

A tri-fluorophoric molecular probe (1) with three different derivatized fluorophores, i.e. anthracene (An), 7-nitrobenz-2-oxa-1,3-diazole (NBD) and rhodamine-B (Rh) appended on to a Tren [tris-(2-aminoethyl)amine] receptor was demonstrated to exhibit metal ion induced ratiometric fluorescence signalling through the initiation of a two-step fluorescence resonance energy transfer (FRET) process owing to a compatible and substantial spectral overlap of electronic absorption and fluorescence of initial donor–intermediate donor/acceptor–final acceptor pairs. The steady-state and time-resolved fluorescence spectral investigation of probes (1a–1f) with individual excitation energy donor (D)/acceptor (A) fluorophores as well as different bi-fluorophoric D–A pairs appended to the same receptor, and comparison with those of 1 revealed a higher energy transfer efficiency between the initial donor (FAn) and the final acceptor (FRh) in the presence of an intermediate (FNBD) fluorophore. The determination of FRET efficiency involving rhodamine-B derivatized systems is complicated as the dynamics and equilibria of their complexation induced spirolactam to ring-opened transformations are driven by various influential parameters; despite this, the FRET efficiencies in 1 and other intermediates were determined and compared under similar conditions for a better comprehension of individual fluorophoric contribution in two-step FRET-based sensing. The selectivity in chelation induced two-step FRET mediated fluorescence signalling in 1 is controlled by a coordination environment and solvent medium; it exhibited Hg2+ ion selective signalling responses in aqueous-MeCN medium while rendering discriminatory responses towards Fe3+/Fe2+ redox couples in MeCN medium.

Graphical abstract: Two-step FRET mediated metal ion induced signalling responses in a probe appended with three fluorophores

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 May 2017, accepted on 09 Jun 2017 and first published on 09 Jun 2017


Article type: Paper
DOI: 10.1039/C7DT01592E
Citation: Dalton Trans., 2017,46, 8975-8991
  •   Request permissions

    Two-step FRET mediated metal ion induced signalling responses in a probe appended with three fluorophores

    B. Biswal, A. Pal and B. Bag, Dalton Trans., 2017, 46, 8975
    DOI: 10.1039/C7DT01592E

Search articles by author

Spotlight

Advertisements