Jump to main content
Jump to site search


A fluorescence ‘turn-on’ chemodosimeter for specific detection of Pd2+ by rhodamine appended Schiff base and its application in live cell imaging

Abstract

A new rhodamine based allyl-ether Schiff base (RD) is spectroscopically characterized and used as colorimetric and fluorimetric sensor. RD acts as turn-on highly selective fluorescent chemosensor for Pd(II) only from the mixture of Pd(0) and Pd(II) in presence of 25 other cations in aqueous-acetonitrile medium. The mechanism of Pd(II) sensing of RD depends on allylphilicity of Pd(II) followed by Pd(II)-triggered terminal (C-O)-allyl cleavage and the opening of the spirolactam ring. Thus the fluorescence enhancement is observed at 563 nm. The proposal has been supported by spectroscopic data (FTIR, 1H NMR, 13C NMR, Mass). The chemical affinity of RD to Pd2+ is visioned efficiently in biological pH range, 6-8. Use of different Pd(II) precursor (PdCl2, Pd(CF3COO)2, Pd(CH3COO)2, Pd(azoimidazole)Cl2, Na2[PdCl4]) shows that the RD exhibits highest emission enhancement for PdCl2. It is again supported by examining the Pd(II) selectivity of non-allylated Rhodamine appended iminephenol (RD/) who has been failed to detect Pd(II). The limit of detection (LOD) of Pd(II) by RD is 50 nM at pH 7.4. Fluorescence microscopic studies help in imaging the cellular uptake of Pd2+ as low as nM level in HCT116 (Human colorectal carcinoma).

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Apr 2017, accepted on 19 Jun 2017 and first published on 19 Jun 2017


Article type: Paper
DOI: 10.1039/C7DT01288H
Citation: Dalton Trans., 2017, Accepted Manuscript
  •   Request permissions

    A fluorescence ‘turn-on’ chemodosimeter for specific detection of Pd2+ by rhodamine appended Schiff base and its application in live cell imaging

    C. Sinha, A. K. Bhanja, S. Mishra and K. Das Saha, Dalton Trans., 2017, Accepted Manuscript , DOI: 10.1039/C7DT01288H

Search articles by author

Spotlight

Advertisements