Jump to main content
Jump to site search

Issue 21, 2017
Previous Article Next Article

A tri-functional vanadium(IV) complex to detect cysteine oxidation

Author affiliations

Abstract

The development of effective molecular probes to detect and image the levels of oxidative stress in cells remains a challenge. Herein we report the design, synthesis and preliminary biological evaluation of a novel optical probe to monitor oxidation of thiol groups in cysteine-based phosphatases (CBPs). Following orthogonal protecting approaches we synthesised a new vanadyl complex designed to bind to CBPs. This complex is functionalised with a well-known dimedone derivative (to covalently trap sulfenic acids, SOHs) and a coumarin-based fluorophore for optical visualization. We show that this new probe efficiently binds to a range of phosphatases in vitro with nanomolar affinity. Moreover, preliminary flow cytometry and microscopy studies in live HCT116 cells show that this probe can successfully image cellular levels of sulfenic acids – one of the species resulting from protein oxidative damage.

Graphical abstract: A tri-functional vanadium(iv) complex to detect cysteine oxidation

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Jan 2017, accepted on 05 May 2017 and first published on 08 May 2017


Article type: Paper
DOI: 10.1039/C7DT00076F
Citation: Dalton Trans., 2017,46, 6994-7004
  •   Request permissions

    A tri-functional vanadium(IV) complex to detect cysteine oxidation

    A. Cilibrizzi, M. Fedorova, J. Collins, R. Leatherbarrow, R. Woscholski and R. Vilar, Dalton Trans., 2017, 46, 6994
    DOI: 10.1039/C7DT00076F

Search articles by author

Spotlight

Advertisements