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Issue 16, 2017
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Transformation of formaldehyde into functional sugars via multi-enzyme stepwise cascade catalysis

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Abstract

The bioconversion of one-carbon compounds into high-value products is important in the energy and chemical fields. In this study, two one-pot multi-enzyme reaction systems were constructed using known formolase and aldolase to synthesise stereodefined functional sugars directly from the prebiotic compound formaldehyde. A stepwise cascade biocatalysis approach was employed, and it decreased the byproduct formation and increased the conversion rate of L-erythrulose from 85% to 91% and L-sorbose from 0 to 68%. This approach was also employed in the whole-cell transformation of low-carbon units, such as formaldehyde, glycolaldehyde, and dihydroxyacetone, and successfully produced 7.56 g L−1L-sorbose. The whole-cell transformation of formaldehyde and dihydroxyacetone resulted in 252 g L−1 (2.21 M) L-erythrulose with a productivity of 126 g L−1 h−1, which is the highest value reported to date.

Graphical abstract: Transformation of formaldehyde into functional sugars via multi-enzyme stepwise cascade catalysis

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Publication details

The article was received on 27 May 2017, accepted on 17 Jul 2017 and first published on 21 Jul 2017


Article type: Communication
DOI: 10.1039/C7CY01062A
Citation: Catal. Sci. Technol., 2017,7, 3459-3463
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    Transformation of formaldehyde into functional sugars via multi-enzyme stepwise cascade catalysis

    J. Yang, S. Sun, Y. Men, Y. Zeng, Y. Zhu, Y. Sun and Y. Ma, Catal. Sci. Technol., 2017, 7, 3459
    DOI: 10.1039/C7CY01062A

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