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Formation of pyrimidine-pyrimidine type DNA intrastrand cross-links: a theoretical verification

Abstract

The pyrimidine-type radicals have been demonstrated to be able to attack their 3' or 5' neighboring purine nucleotides forming diverse DNA intrastrand cross-links, but whether or not these radicals can attack their surrounding pyrimidine nucleotides forming the pyrimidine-pyrimidine type DNA intrastrand cross-links remains unclear. To resolve this question, probable additions of the uracil-5-methyl (UCH2) radical to the C5=C6 double bond of its 3'/5' neighboring pyrimidine nucleotides in the four models, 5'-T(UCH2)-3', 5'-C(UCH2)-3', 5'-(UCH2)T-3', and 5'-(UCH2)C-3', are explored in the present work employing density functional theory (DFT) methods. The C6 site of its 5' neighboring thymidine is the preferable target for UCH2 radical addition, while additions of UCH2 radical to the C6 and C5 sites of its 5' neighboring deoxycytidine are found to be competitive reactions. The UCH2 radical can react with both the C6 and C5 sites of its 3' neighboring pyrimidine nucleotides, but the efficiencies of these reactions are predicted to be much lower than those of the corresponding addition reactions to its 5' neighboring pyrimidine nucleotides, indicating the existence of an obvious sequence effect. All addition products could be finally transformed into closed-shell intrastrand cross-links, the molecular masses of which are found to be exactly the same to certain MS values determined in a recent study of X-irradiated deoxygenated aqueous solution of calf thymus DNA. The present study thus not only definitely corroborates that the reactive UCH2 radical can attack its 3'/5' neighboring pyrimidine nucleotides forming several pyrimidine-pyrimidine type DNA intrastrand cross-links, but also provides a plausible explanation for the identities of these structurally unknown intrastrand cross-links.

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Publication details

The article was accepted on 29 Sep 2017 and first published on 03 Oct 2017


Article type: Paper
DOI: 10.1039/C7CP06452G
Citation: Phys. Chem. Chem. Phys., 2017, Accepted Manuscript
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    Formation of pyrimidine-pyrimidine type DNA intrastrand cross-links: a theoretical verification

    S. Wang, M. Zhang, P. Liu, S. Xie, F. Cheng and L. Wang, Phys. Chem. Chem. Phys., 2017, Accepted Manuscript , DOI: 10.1039/C7CP06452G

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