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Issue 45, 2017
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Intersegmental transfer of proteins between DNA regions in the presence of crowding

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Abstract

Intersegmental transfer that involves direct relocation of a DNA-binding protein from one nonspecific DNA site to another was previously shown to contribute to speeding up the identification of the DNA target site. This mechanism is promoted when the protein is composed of at least two domains that have different DNA binding affinities and thus show a degree of mobility. In this study, we investigate the effect of particle crowding on the ability of a multi-domain protein to perform intersegmental transfer. We show that although crowding conditions often favor 1D diffusion of proteins along DNA over 3D diffusion, relocation of one of the tethered domains to initiate intersegmental transfer is possible even under crowding conditions. The tendency to perform intersegmental transfer by a multi-domain protein under crowding conditions is much higher for larger crowding particles than smaller ones and can be even greater than under no-crowding conditions. We report that the asymmetry of the two domains is even magnified by the crowders. The observations that crowding supports intersegmental transfer serve as another example that in vivo complexity does not necessarily slow down DNA search kinetics by proteins.

Graphical abstract: Intersegmental transfer of proteins between DNA regions in the presence of crowding

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Publication details

The article was received on 03 Aug 2017, accepted on 16 Oct 2017 and first published on 16 Oct 2017


Article type: Paper
DOI: 10.1039/C7CP05251K
Citation: Phys. Chem. Chem. Phys., 2017,19, 30562-30569
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    Intersegmental transfer of proteins between DNA regions in the presence of crowding

    D. Krepel and Y. Levy, Phys. Chem. Chem. Phys., 2017, 19, 30562
    DOI: 10.1039/C7CP05251K

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